File:The-Intracellular-Bacteria-Chlamydia-Hijack-Peroxisomes-and-Utilize-Their-Enzymatic-Capacity-to-pone.0086196.s002.ogv

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Original file ‎(Ogg Theora video file, length 20 s, 624 × 750 pixels, 265 kbps, file size: 646 KB)

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English: HeLa cells were transfected with GFP (green) to illuminate the entire cell except for the Chlamydia inclusion and were infected with C. trachomatis L2 for 20 h. Bacteria were labeled with anti-Hsp60 antibody (blue) and peroxisomes with anti-ALDP (red). A z-dimension series of images was acquired every 0.2 µm with Metamorph software and a 3D deconvolution microscope (DM-RXA2, Leica) equipped with a 100x Plan Apo NA 1.4 oil objective and a cooled charge-coupled device (CCD) camera (Roper CoolSnap HQ2). Z-dimension positioning was accomplished with a piezoelectric motor (LVDT, Physik Instrument). The images were then deconvoluted. Adjacent optical slices are displayed as adjacent frames in the movie.
Date
Source Movie S1 from Boncompain G, Muller C, Meas-Yedid V, Schmitt-Kopplin P, Lazarow P, Subtil A (2014). "The Intracellular Bacteria Chlamydia Hijack Peroxisomes and Utilize Their Enzymatic Capacity to Produce Bacteria-Specific Phospholipids". PLOS ONE. DOI:10.1371/journal.pone.0086196. PMC: 3900481.
Author Boncompain G, Muller C, Meas-Yedid V, Schmitt-Kopplin P, Lazarow P, Subtil A
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current23:13, 25 January 201420 s, 624 × 750 (646 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 720P 126 kbps Completed 20:13, 21 October 2018 3.0 s
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VP9 480P 60 kbps Completed 20:13, 21 October 2018 4.0 s
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VP9 360P 32 kbps Completed 20:13, 21 October 2018 3.0 s
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VP9 240P 16 kbps Completed 20:13, 21 October 2018 2.0 s
Streaming 240p (VP9) 16 kbps Completed 02:25, 7 February 2024 1.0 s
WebM 360P 188 kbps Completed 23:13, 25 January 2014 2.0 s
Streaming 144p (MJPEG) 41 kbps Completed 12:00, 9 November 2023 1.0 s

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