File:The-In-Vivo-Kinetics-of-RNA-Polymerase-II-Elongation-during-Co-Transcriptional-Splicing-pbio.1000573.s012.ogv

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The-In-Vivo-Kinetics-of-RNA-Polymerase-II-Elongation-during-Co-Transcriptional-Splicing-pbio.1000573.s012.ogv ‎(Ogg Theora video file, length 5.8 s, 320 × 336 pixels, 115 kbps, file size: 82 KB)

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English: Transcriptional activation of E3 (top) and E6 (bottom) cells. The DNA locus is tagged with CFP-LacI and the cytoplasmic peroxisomes are also seen in CFP. The mRNA is tagged with YFP-MS2. As transcriptional induction proceeds, the transcription sites appear and then mRNAs disperse into the nucleoplasm. CFP-peroxisomes appear at later times. Since the videos were acquired in 3D and the z-stacks covered the cell volumes, we could show the nuclear CFP-LacI locus in the beginning of the videos and then later shift to the cytoplasmic plane that best shows the CFP-peroxisomes. Cells were imaged 15 min after dox addition and the time in the videos is measured from dox addition. E3, imaged every 15 min; E6, imaged every 20 min.
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Source Video S1 from Brody Y, Neufeld N, Bieberstein N, Causse S, Böhnlein E, Neugebauer K, Darzacq X, Shav-Tal Y (2011). "The In Vivo Kinetics of RNA Polymerase II Elongation during Co-Transcriptional Splicing". PLOS Biology. DOI:10.1371/journal.pbio.1000573. PMID 21264352. PMC: 3019111.
Author Brody Y, Neufeld N, Bieberstein N, Causse S, Böhnlein E, Neugebauer K, Darzacq X, Shav-Tal Y
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current00:52, 31 October 20125.8 s, 320 × 336 (82 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 31 kbps Completed 20:03, 21 October 2018 2.0 s
Streaming 240p (VP9) 31 kbps Completed 17:04, 17 December 2023 1.0 s
WebM 360P 58 kbps Completed 15:37, 2 December 2023 0.0 s
Streaming 144p (MJPEG) 123 kbps Completed 11:47, 9 November 2023 1.0 s

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