File:The-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s007.ogv

English: Double-occupancy L. major PVs present dynamic lysotracker clusters in the interface between amastigotes. (A) Multidimensional imaging of dividing L. major-DsRed2 amastigotes (red) hosted by a macrophage loaded with Lysotracker (green). Polarized Lysotracker clusters are observed in amastigotes prior to division; after division, clusters are found between two divided parasites. Image acquisition started after 3 h of infection, and the time of acquisition is shown as d:hh:mm. (B–C) The Lysotracker cluster between dividing parasites was observed in multidimensional images. The attribution of an isosurface to the Lysotracker-positive interface permitted the measurement of its volume at each time point. The isosurface contains statistic-based color information ranging from cyan (smaller volumes) to magenta (larger volumes). Image acquisition started after 2 h of infection, and the time of acquisition is shown as d:hh:mm. (D–E) Another multidimensional image acquired in shorter time intervals, showing the lysotracker-positive interface between dividing parasites. Image acquisition started after 48 h of infection, and the time of acquisition is shown as hh:mm.