File:The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S9.ogv
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The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S9.ogv (Ogg Theora video file, length 19 s, 352 × 288 pixels, 95 kbps, file size: 221 KB)
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[edit]DescriptionThe-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S9.ogv |
English: Additional file 9: Video S6/Figure 4. APPsw is not cleared in the Golgi apparatus. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPPsw-paGFP and were treated with 66 μM nocodazole and 10 μM cytochalasin. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPPsw-paGFP can be seen accumulating in the Golgi. |
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Source | Video file from Tam J, Seah C, Pasternak S (2014). "The Amyloid Precursor Protein is rapidly transported from the Golgi apparatus to the lysosome and where it is processed into beta-amyloid". Molecular Brain. DOI:10.1186/s13041-014-0054-1. PMID 25085554. PMC: 4237969. | ||
Author | Tam J, Seah C, Pasternak S | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:06, 22 November 2014 | 19 s, 352 × 288 (221 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 9: Video S6/Figure 4. |
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Author | Tam J, Seah C, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | APPsw is not cleared in the Golgi apparatus. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPPsw-paGFP and were treated with 66 μM nocodazole and 10 μM cytochalasin. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPPsw-paGFP can be seen accumulating in the Golgi. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |