File:The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S6.ogv
The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S6.ogv (Ogg Theora video file, length 35 s, 352 × 288 pixels, 94 kbps, file size: 404 KB)
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[edit]DescriptionThe-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S6.ogv |
English: Additional file 6: Video S3/Figure 3. APP processing in the lysosome is blocked by Chloroquine in the lysosome. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPP-paGFP. Cells were pretreated with 100 μM chloroquine 30 minutes before imaging. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPP-paGFP can be seen accumulating in lysosomes. |
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Source | Video file from Tam J, Seah C, Pasternak S (2014). "The Amyloid Precursor Protein is rapidly transported from the Golgi apparatus to the lysosome and where it is processed into beta-amyloid". Molecular Brain. DOI:10.1186/s13041-014-0054-1. PMID 25085554. PMC: 4237969. | ||
Author | Tam J, Seah C, Pasternak S | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 12:05, 22 November 2014 | 35 s, 352 × 288 (404 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 6: Video S3/Figure 3. |
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Author | Tam J, Seah C, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | APP processing in the lysosome is blocked by Chloroquine in the lysosome. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPP-paGFP. Cells were pretreated with 100 μM chloroquine 30 minutes before imaging. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPP-paGFP can be seen accumulating in lysosomes. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |