File:The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S3.ogv
The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S3.ogv (Ogg Theora video file, length 45 s, 352 × 288 pixels, 154 kbps, file size: 842 KB)
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[edit]DescriptionThe-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S3.ogv |
English: Additional file 3: Video S1/Figure 1. APP is trafficked rapidly to the lysosome and cleared. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPP-paGFP. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. |
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Source | Video file from Tam J, Seah C, Pasternak S (2014). "The Amyloid Precursor Protein is rapidly transported from the Golgi apparatus to the lysosome and where it is processed into beta-amyloid". Molecular Brain. DOI:10.1186/s13041-014-0054-1. PMID 25085554. PMC: 4237969. | ||
Author | Tam J, Seah C, Pasternak S | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:04, 22 November 2014 | 45 s, 352 × 288 (842 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 3: Video S1/Figure 1. |
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Author | Tam J, Seah C, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | APP is trafficked rapidly to the lysosome and cleared. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPP-paGFP. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |