File:The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S11.ogv
The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S11.ogv (Ogg Theora video file, length 34 s, 352 × 288 pixels, 144 kbps, file size: 599 KB)
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[edit]DescriptionThe-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S11.ogv |
English: Additional file 11: Video S8/Figure 4. APPSw processing in the lysosome is blocked by L685, 458; γ-cleavage occurs in the lysosome. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPPsw-paGFP and were treated with 0.5 μM L685, 458 overnight. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPPsw-paGFP can be seen accumulating in lysosomes. (MOV 449 kb) |
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Source | Video file from Tam J, Seah C, Pasternak S (2014). "The Amyloid Precursor Protein is rapidly transported from the Golgi apparatus to the lysosome and where it is processed into beta-amyloid". Molecular Brain. DOI:10.1186/s13041-014-0054-1. PMID 25085554. PMC: 4237969. | ||
Author | Tam J, Seah C, Pasternak S | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:06, 22 November 2014 | 34 s, 352 × 288 (599 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 11: Video S8/Figure 4. |
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Author | Tam J, Seah C, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | APPSw processing in the lysosome is blocked by L685, 458; γ-cleavage occurs in the lysosome. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPPsw-paGFP and were treated with 0.5 μM L685, 458 overnight. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPPsw-paGFP can be seen accumulating in lysosomes. (MOV 449 kb) |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2014 |