File:The-3-Untranslated-Region-of-the-Cyclin-B-mRNA-Is-Not-Sufficient-to-Enhance-the-Synthesis-of-Cyclin-pone.0074379.s002.ogv
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The-3-Untranslated-Region-of-the-Cyclin-B-mRNA-Is-Not-Sufficient-to-Enhance-the-Synthesis-of-Cyclin-pone.0074379.s002.ogv (Ogg Theora video file, length 11 s, 324 × 172 pixels, 336 kbps, file size: 443 KB)
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[edit]DescriptionThe-3-Untranslated-Region-of-the-Cyclin-B-mRNA-Is-Not-Sufficient-to-Enhance-the-Synthesis-of-Cyclin-pone.0074379.s002.ogv |
English: Degradation and synthesis of the double-chimeric cyclin B reporter throughout the cell cycle by live-cell imaging at the single-cell level in U2Os cells. The movie depicts two single cells that express the CYS reporter molecule and were stained with BG430. An overlay of YFP (cyclin B expression), BG430 (cyclin B degradation) and mCherry (chromosomes) fluorescence is presented. Both cells exhibit a rapid decline in YFP and BG430 fluorescence intensity (turquoise fluorescence) during mitotic exit. Over time, all daughter cells show a steady increase in YFP fluorescence intensity (green fluorescence) indicating re-accumulation of the CYS reporter molecule. |
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Source | Movie S1 from Schnerch D, Follo M, Felthaus J, Engelhardt M, Wasch R (2013). "The 3' Untranslated Region of the Cyclin B mRNA Is Not Sufficient to Enhance the Synthesis of Cyclin B during a Mitotic Block in Human Cells". PLOS ONE. DOI:10.1371/journal.pone.0074379. PMID 24058555. PMC: 3772928. | ||
Author | Schnerch D, Follo M, Felthaus J, Engelhardt M, Wasch R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 18:58, 25 September 2013 | 11 s, 324 × 172 (443 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Schnerch D, Follo M, Felthaus J, Engelhardt M, Wasch R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Degradation and synthesis of the double-chimeric cyclin B reporter throughout the cell cycle by live-cell imaging at the single-cell level in U2Os cells. The movie depicts two single cells that express the CYS reporter molecule and were stained with BG430. An overlay of YFP (cyclin B expression), BG430 (cyclin B degradation) and mCherry (chromosomes) fluorescence is presented. Both cells exhibit a rapid decline in YFP and BG430 fluorescence intensity (turquoise fluorescence) during mitotic exit. Over time, all daughter cells show a steady increase in YFP fluorescence intensity (green fluorescence) indicating re-accumulation of the CYS reporter molecule. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |