File:Terminal-Platelet-Production-is-Regulated-by-Von-Willebrand-Factor-pone.0063810.s005.ogv

English: Adhesion of human platelets. Human platelets from a healthy volunteer were perfused at a shear rate of 1800 s−1 for 18 min on VWF-coated coverslips and platelets were visualized in real-time on the VWF surface. Different morphologies of proplatelets are present. For example, in the upper part of the field, during the first 18 seconds of the video, notice an elongated newly formed platelet that translocates along the field and folds like a knot, then unfolds to appear as an extended platelet. In the upper third of the field, entering at time 37″ until 57″, notice how another newly formed platelet displays different shapes during its translocation on the surface. Digital images were recorded at 0.25 image/s using Replay software (Microvision Instruments). For visualization of the movie, frames were grabbed so as to accelerate to a velocity of 10 images/s. Bar represents 20 µm.