File:Terminal-Platelet-Production-is-Regulated-by-Von-Willebrand-Factor-pone.0063810.s003.ogv
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Terminal-Platelet-Production-is-Regulated-by-Von-Willebrand-Factor-pone.0063810.s003.ogv (Ogg Theora video file, length 31 s, 450 × 339 pixels, 332 kbps, file size: 1.23 MB)
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[edit]DescriptionTerminal-Platelet-Production-is-Regulated-by-Von-Willebrand-Factor-pone.0063810.s003.ogv |
English: Adhesion of Vwf −/− platelets. Mouse platelets were perfused at a shear rate of 1800 s−1 for 35 min on VWF-coated coverslips and adherent platelets were visualized on the VWF surface. Digital images were recorded at 0.25 image/s using “Replay” and “Histolab” software. For visualization, “Archimed” software (Microvision Instruments) was used to grab frames and record at a velocity of 10 images/s (40-fold acceleration). The set-up allows analyzing the changes of platelet morphology during their crossing of one field (an event that may extend up to 10 min). The release of a small discoid platelet from a proplatelet can be visualized in real-time: in the lower third of the image on the left, entering the field at 16′54″ is a dumbbell-shaped proplatelet (arrowhead) with a thin extension at its rear; the proplatelet translocates along the field and its extension corresponds to a small platelet (arrow at 18′06″), which is released at 20′42″ (arrow) from the original proplatelet (arrowhead). Bar is 20 µm. |
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Source | Video S1 from Poirault-Chassac S, Nguyen K, Pietrzyk A, Casari C, Veyradier A, Denis C, Baruch D (2013). "Terminal Platelet Production is Regulated by Von Willebrand Factor". PLOS ONE. DOI:10.1371/journal.pone.0063810. PMID 23737952. PMC: 3667798. | ||
Author | Poirault-Chassac S, Nguyen K, Pietrzyk A, Casari C, Veyradier A, Denis C, Baruch D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:07, 13 June 2013 | 31 s, 450 × 339 (1.23 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Poirault-Chassac S, Nguyen K, Pietrzyk A, Casari C, Veyradier A, Denis C, Baruch D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |
Categories:
- Extracellular matrix
- Extracellular matrix adhesions
- Coagulation factors
- Biological fluid mechanics
- Videos of developmental biology
- Molecular development
- Adhesion molecules
- Animal genetics
- Molecular cell biology
- Videos of cell types
- Videos of stem cells
- Hematopoietic stem cells
- Videos of blood cells
- Bone marrow cells
- Endothelial cells
- Cell adhesion
- Vascular biology
- Coagulation disorders
- Media from PLOS ONE