File:Targeting-Cx43-and-N-Cadherin-Which-Are-Abnormally-Upregulated-in-Venous-Leg-Ulcers-Influences-pone.0037374.s007.ogv
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Original file (Ogg Theora video file, length 7.6 s, 846 × 430 pixels, 567 kbps, file size: 529 KB)
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[edit]DescriptionTargeting-Cx43-and-N-Cadherin-Which-Are-Abnormally-Upregulated-in-Venous-Leg-Ulcers-Influences-pone.0037374.s007.ogv |
English: Knockdown of Cx43 accelerates the rate of fibroblasts migration. Fibroblasts stably transfected with Cx43shRNA or p.Sup were monitored for 3 h and time-lapse images were taken at 5 min intervals. Cells transfected with the Cx43shRNA migrated faster than their respective controls, further confirming the close relationship between Cx43 expression levels and the rate of migration in fibroblasts. Cx43shRNA-transfected cells also displayed the same phenotype observed in Cx43asODN treated fibroblasts, characterized by the formation of extensive lamellipodia during migration. |
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Source | Movie S2 from Mendoza-Naranjo A, Cormie P, Serrano A, Hu R, O'Neill S, Wang C, Thrasivoulou C, Power K, White A, Serena T, Phillips A, Becker D (2012). "Targeting Cx43 and N-Cadherin, Which Are Abnormally Upregulated in Venous Leg Ulcers, Influences Migration, Adhesion and Activation of Rho GTPases". PLOS ONE. DOI:10.1371/journal.pone.0037374. PMID 22615994. PMC: 3352877. | ||
Author | Mendoza-Naranjo A, Cormie P, Serrano A, Hu R, O'Neill S, Wang C, Thrasivoulou C, Power K, White A, Serena T, Phillips A, Becker D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 04:46, 14 November 2012 | 7.6 s, 846 × 430 (529 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Mendoza-Naranjo A, Cormie P, Serrano A, Hu R, O'Neill S, Wang C, Thrasivoulou C, Power K, White A, Serena T, Phillips A, Becker D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Knockdown of Cx43 accelerates the rate of fibroblasts migration. Fibroblasts stably transfected with Cx43shRNA or p.Sup were monitored for 3 h and time-lapse images were taken at 5 min intervals. Cells transfected with the Cx43shRNA migrated faster than their respective controls, further confirming the close relationship between Cx43 expression levels and the rate of migration in fibroblasts. Cx43shRNA-transfected cells also displayed the same phenotype observed in Cx43asODN treated fibroblasts, characterized by the formation of extensive lamellipodia during migration. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |