File:Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv006.ogv
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Surface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv006.ogv (Ogg Theora video file, length 20 s, 320 × 256 pixels, 250 kbps, file size: 611 KB)
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[edit]DescriptionSurface-Anchored-Monomeric-Agonist-pMHCs-Alone-Trigger-TCR-with-High-Sensitivity-pbio.0060043.sv006.ogv |
English: T cells Do Not Adhere to Bilayers without Anchored ICAM-1 POPC bilayers with 5 mol% DOPE-biotin, without anchored (ICAM-1)2-SA, were tilted at a very small angle so that, without adhesion, T cells moved slowly on the surface due to gravity. T cells were imaged in buffer containing 0.25 mM Ca2+ and 0.25 mM Mn2+ to activate LFA-1 and promote adhesion. Movement of T cells was captured with DIC bright field imaging at 5-s intervals. The speed of the video is 50 times real time. |
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Source | Video S6 from Ma Z, Sharp K, Janmey P, Finkel T (2008). "Surface-Anchored Monomeric Agonist pMHCs Alone Trigger TCR with High Sensitivity". PLOS Biology. DOI:10.1371/journal.pbio.0060043. PMID 18303949. PMC: 2253636. | ||
Author | Ma Z, Sharp K, Janmey P, Finkel T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 14:48, 16 November 2012 | 20 s, 320 × 256 (611 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | T cells Do Not Adhere to Bilayers without Anchored ICAM-1 |
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Author | Ma Z, Sharp K, Janmey P, Finkel T |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | POPC bilayers with 5 mol% DOPE-biotin, without anchored (ICAM-1)2-SA, were tilted at a very small angle so that, without adhesion, T cells moved slowly on the surface due to gravity. T cells were imaged in buffer containing 0.25 mM Ca2+ and 0.25 mM Mn2+ to activate LFA-1 and promote adhesion. Movement of T cells was captured with DIC bright field imaging at 5-s intervals. The speed of the video is 50 times real time. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-02 |