File:Substrate-Dependent-Assembly-of-the-Tat-Translocase-as-Observed-in-Live-Escherichia-coli-Cells-pone.0069488.s005.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Substrate-Dependent-Assembly-of-the-Tat-Translocase-as-Observed-in-Live-Escherichia-coli-Cells-pone.0069488.s005.ogv ‎(Ogg Theora video file, length 10 s, 145 × 121 pixels, 77 kbps, file size: 94 KB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary

[edit]
Description
English: Dynamics of TatA-GFP clusters. BL21(DE3) cells expressing TatA-GFP from plasmid pPR1 were grown and induced as described in Material and Methods. Fluorescence microscopy was performed using a Zeiss Axio Observer Z1 microscope and a 100x objective with a numerical aperture (NA) of 1.45, using a TIRF setup from Visitron (Munich, Germany). The movie was recorded with an Evolve EM-CCD camera (Photometrix) and processed with Metamorph 6.3 (Universal Imaging Corp.) and ImageJ 1.44 software. GFPmut1 was excited by a laser of 488 nm wavelength. The movie (5 frames/sec) shows the real-time movement of TatA-GFP in two cells. At their distal poles, both cells show a dynamic appearance and localization of TatA-GFP clusters including apparent fusions of two or more clusters. At the presumed division site of the left cell a new cluster is formed from several foci that originate from positions in the middle of the cell.
Date
Source Movie S1 from Rose P, Frobel J, Graumann P, Muller M (2013). "Substrate-Dependent Assembly of the Tat Translocase as Observed in Live Escherichia coli Cells". PLOS ONE. DOI:10.1371/journal.pone.0069488. PMID 23936332. PMC: 3732296.
Author Rose P, Frobel J, Graumann P, Muller M
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
PLOS
PLOS
This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current13:33, 22 March 201710 s, 145 × 121 (94 KB)Revent (talk | contribs)attempt to fix transcodes
04:42, 18 August 201310 s, 145 × 121 (55 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 240P 22 kbps Completed 21:04, 16 July 2024 1.0 s
Streaming 240p (VP9) 22 kbps Completed 01:36, 17 December 2023 1.0 s
WebM 360P 25 kbps Completed 13:36, 2 December 2023 0.0 s
Streaming 144p (MJPEG) 97 kbps Completed 16:37, 19 November 2023 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Substrate-Dependent-Assembly-of-the-Tat-Translocase-as-Observed-in-Live-Escherichia-coli-Cells-pone.0069488.s005.ogv&oldid=476775052"