File:Stage-Specific-Changes-in-the-Water-Na+-Cl--and-K+-Contents-of-Organelles-during-Apoptosis-pone.0148727.s010.ogv

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Stage-Specific-Changes-in-the-Water-Na+-Cl--and-K+-Contents-of-Organelles-during-Apoptosis-pone.0148727.s010.ogv (Ogg Theora video file, length 3.3 s, 472 × 456 pixels, 16.85 Mbps, file size: 6.69 MB)

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English: Simultaneous 3D localization of activated caspase-3 (actCASP3), cleaved PARP (clePARP) and H2B-GFP, demonstrating that ST 2 to ST 5 cells have entered apoptosis. Anti-activated caspase-3 and anti-cleaved PARP antibodies were used to label fixed HeLa cells stably expressing H2B-GFP after the induction of apoptosis by incubation with 500 ng/mL AMD for 7 h 15 minutes. For each stage of apoptosis and stage 0, Z-stacks were processed for a simultaneous 3D transparent volume rendering of actCASP3 (blue), clePARP (red) and H2B-GFP (green) which was then fully rotated. The six cells (ST1 to ST5 and ST0) are shown in the same window to improve comparison of actCASP3, clePARP and H2B-GFP localization.
Date
Source S7 Movie from Nolin F, Michel J, Wortham L, Tchelidze P, Banchet V, Lalun N, Terryn C, Ploton D (2016). "Stage-Specific Changes in the Water, Na+, Cl- and K+ Contents of Organelles during Apoptosis, Demonstrated by a Targeted Cryo Correlative Analytical Approach". PLOS ONE. DOI:10.1371/journal.pone.0148727. PMID 26866363. PMC: 4807926.
Author Nolin F, Michel J, Wortham L, Tchelidze P, Banchet V, Lalun N, Terryn C, Ploton D
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current13:56, 13 October 20163.3 s, 472 × 456 (6.69 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 294 kbps Completed 11:19, 20 October 2018 5.0 s
VP9 240P 183 kbps Completed 11:19, 20 October 2018 4.0 s
WebM 360P 522 kbps Completed 13:56, 13 October 2016 5.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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