File:Spreading-of-a-Prion-Domain-from-Cell-to-Cell-by-Vesicular-Transport-in-Caenorhabditis-elegans-pgen.1003351.s015.ogv
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Spreading-of-a-Prion-Domain-from-Cell-to-Cell-by-Vesicular-Transport-in-Caenorhabditis-elegans-pgen.1003351.s015.ogv (Ogg Theora video file, length 8.1 s, 250 × 250 pixels, 866 kbps, file size: 851 KB)
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[edit]DescriptionSpreading-of-a-Prion-Domain-from-Cell-to-Cell-by-Vesicular-Transport-in-Caenorhabditis-elegans-pgen.1003351.s015.ogv |
English: R2E2i::RFP containing vesicles are able to move from C. elegans intestinal cells into non-expressing tissues. Nematodes expressing R2E2i::RFP were mounted and movies were taken using a Zeiss LSM510 confocal microscope. 161 frames were taken with a speed of 0.5 fps. Video is played at 20 fps. As the low fluorescence of released vesicles reaches the detection limit of the microscope, the RFP channel was overexposed to better show vesicular structures outside of the intestine, which results in a low video quality. Figure S5 shows a representative picture of this movie and outlines the tissues and highlights the released vesicles. |
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Source | Video S7 from Nussbaum-Krammer C, Park K, Li L, Melki R, Morimoto R (2013). "Spreading of a Prion Domain from Cell-to-Cell by Vesicular Transport in Caenorhabditis elegans". PLOS Genetics. DOI:10.1371/journal.pgen.1003351. PMID 23555277. PMC: 3610634. | ||
Author | Nussbaum-Krammer C, Park K, Li L, Melki R, Morimoto R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 02:35, 7 April 2013 | 8.1 s, 250 × 250 (851 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Nussbaum-Krammer C, Park K, Li L, Melki R, Morimoto R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | R2E2i::RFP containing vesicles are able to move from C. elegans intestinal cells into non-expressing tissues. Nematodes expressing R2E2i::RFP were mounted and movies were taken using a Zeiss LSM510 confocal microscope. 161 frames were taken with a speed of 0.5 fps. Video is played at 20 fps. As the low fluorescence of released vesicles reaches the detection limit of the microscope, the RFP channel was overexposed to better show vesicular structures outside of the intestine, which results in a low video quality. Figure S5 shows a representative picture of this movie and outlines the tissues and highlights the released vesicles. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-03 |