File:Specific-Visualization-of-Nitric-Oxide-in-the-Vasculature-with-Two-Photon-Microscopy-Using-a-Copper-pone.0075331.s007.ogv

English: NO production dynamics from every layer of the vessel (the overall vessel wall volume, changes in its cellular structure); direct visualization of NO signal in SMCs at the medial layer and in ECs at the intimal layer of the vessel wall. In the movie, part of the murine carotid artery can be observed aligned horizontally that has been incubated with Cu 2FL2E stimulated with ACh, then scanning using TPLSM takes place from the adventitial side to luminal side. Fibroblasts along with collagen (in blue) can be seen at the adventitia, with progression to medial layer, “spindle shaped” SMCs (green) aligned vertically becomes apparent due to NO signal and following to intimal side, ECs aligned horizontally can be seen to produce NO signal (in green). In this movie, z-stack of individual 42 sections has been taken at a depth of 39.5 µm, with each step size of 0.99 µm. The scanning speed was 100Hz with optical zoom of 3.3.