File:Specialized-Peptidoglycan-Hydrolases-Sculpt-the-Intra-bacterial-Niche-of-Predatory-Bdellovibrio-and-ppat.1002524.s024.ogv
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Specialized-Peptidoglycan-Hydrolases-Sculpt-the-Intra-bacterial-Niche-of-Predatory-Bdellovibrio-and-ppat.1002524.s024.ogv (Ogg Theora video file, length 2.0 s, 266 × 263 pixels, 70 kbps, file size: 17 KB)
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[edit]DescriptionSpecialized-Peptidoglycan-Hydrolases-Sculpt-the-Intra-bacterial-Niche-of-Predatory-Bdellovibrio-and-ppat.1002524.s024.ogv |
English: Time lapse movie showing E. coli TOP10 cells expressing wild type Bd3459 protein on a M-medium-agarose pad from 0–6 hours after inducing protein expression. The movie shows several cells that initially being to elongate, but after approximately 2 hours have neatly rounded up (reminiscent of bdelloplasts) as they are osmotically supported by the hypertonic medium. Eventually the cells become larger and more irregular, but this could have been due to the tonicity of the media changing as the agarose pad dried out. Video is at 3 frames per second. |
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Source | Video S15 from Lerner T, Lovering A, Bui N, Uchida K, Aizawa S, Vollmer W, Sockett R (2012). "Specialized Peptidoglycan Hydrolases Sculpt the Intra-bacterial Niche of Predatory Bdellovibrio and Increase Population Fitness". PLOS Pathogens. DOI:10.1371/journal.ppat.1002524. PMID 22346754. PMC: 3276566. | ||
Author | Lerner T, Lovering A, Bui N, Uchida K, Aizawa S, Vollmer W, Sockett R | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 09:13, 16 November 2012 | 2.0 s, 266 × 263 (17 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Lerner T, Lovering A, Bui N, Uchida K, Aizawa S, Vollmer W, Sockett R |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Time lapse movie showing E. coli TOP10 cells expressing wild type Bd3459 protein on a M-medium-agarose pad from 0?6 hours after inducing protein expression. The movie shows several cells that initially being to elongate, but after approximately 2 hours have neatly rounded up (reminiscent of bdelloplasts) as they are osmotically supported by the hypertonic medium. Eventually the cells become larger and more irregular, but this could have been due to the tonicity of the media changing as the agarose pad dried out. Video is at 3 frames per second. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-02 |