File:Spatial-separation-and-bidirectional-trafficking-of-proteins-using-a-multi-functional-reporter-1471-2121-9-17-S2.ogv
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[edit]DescriptionSpatial-separation-and-bidirectional-trafficking-of-proteins-using-a-multi-functional-reporter-1471-2121-9-17-S2.ogv |
English: Real-time translocation of proteins using HaloTag Technology. Timelapse imaging of live U2OS cells sequentially labeled with HaloTag 488 and TMR ligands shows real-time translocation of both protein pools. After labeling, time 0 shows spatial separation of the green surface protein around the red internal protein. Imaging every 20 minutes over 17 hours first shows the green surface pool internalizing and the red internal pool trafficking to the surface, then the original green surface pool inside and the red pool on the membrane, and finally the red surface pool internalizing. |
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Date | |||
Source | Svendsen S, Zimprich C, McDougall M, Klaubert D, Los G (2008). "Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter". BMC Cell Biology. DOI:10.1186/1471-2121-9-17. PMID 18384686. PMC: 2359743. | ||
Author | Svendsen S, Zimprich C, McDougall M, Klaubert D, Los G | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 05:18, 2 July 2013 | 15 s, 748 × 748 (1.91 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Svendsen S, Zimprich C, McDougall M, Klaubert D, Los G |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Real-time translocation of proteins using HaloTag Technology. Timelapse imaging of live U2OS cells sequentially labeled with HaloTag 488 and TMR ligands shows real-time translocation of both protein pools. After labeling, time 0 shows spatial separation of the green surface protein around the red internal protein. Imaging every 20 minutes over 17 hours first shows the green surface pool internalizing and the red internal pool trafficking to the surface, then the original green surface pool inside and the red pool on the membrane, and finally the red surface pool internalizing. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008 |