File:Slow-angled-descent-forepaw-grasping-(SLAG)-an-innate-behavioral-task-for-identification-of-1744-9081-9-35-S1.ogv

English: Supplemental Video 1. Demonstration of the slow angled-descent forepaw grasping (SLAG) test for identification of sighted mice. The left side of the screen shows a SLAG(+) mouse, while the right side shows a SLAG(−) mouse. Approximately 15–30 cm above an illuminated wire-bar cage lid, each mouse is suspended by the tail with its ventral aspect oriented toward the same side as the raised edge of the lid (from the viewer’s perspective, the right side). The mouse is slowly lowered over and past the wire lid, and in this orientation, the wire lid remains within the mouse’s field of view during descent. Upon nearly passing the edge of the wire lid, the SLAG(+) mouse (left) stretches forth its forepaws in a sustained fashion toward the wire lid, whereas the SLAG(−) mouse (right) does not. Regardless of its performance, each mouse is briefly placed on the wire lid (not shown for the SLAG(−) mouse on the right). The test is immediately repeated, but with the mouse starting in the opposite horizontal orientation. Upon descent, the wire lid becomes excluded from the mouse’s field of view when the mouse passes the raised edge, and the SLAG(+) mouse (left) twists around to extend its forepaws toward the wire lid in a sustained fashion, whereas the SLAG(−) mouse (right) does not. The video bears the watermark of Wondershare™, the freeware used to convert the video into a format compatible with the memory and accessibility requirements of the journal.