File:Single-molecule-imaging-reveals-a-major-role-for-diffusion-in-the-exploration-of-ciliary-space-by-elife00654v001.ogv
Single-molecule-imaging-reveals-a-major-role-for-diffusion-in-the-exploration-of-ciliary-space-by-elife00654v001.ogv (Ogg Theora video file, length 8.5 s, 258 × 258 pixels, 30 kbps, file size: 31 KB)
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[edit]DescriptionSingle-molecule-imaging-reveals-a-major-role-for-diffusion-in-the-exploration-of-ciliary-space-by-elife00654v001.ogv |
English: Live cell imaging of a single ciliary SSTR3 molecule. IMCD3 cells stably expressing AP-SSTR3-GFP (pseudo-colored red) were transfected with BirA-ER to biotinylate AP-SSTR3-GFP. Biotinylated SSTR3 was detected with mSA-Alexa647 (mSA, pseudo-colored green). The tip (T) and the base (B) of the cilium are indicated. Scale bar, 2 μm.
DOI: https://dx.doi.org/10.7554/eLife.00654.007 |
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Source | Video 1. from Ye F, Breslow D, Koslover E, Spakowitz A, Nelson W, Nachury M (2013). "Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors". eLife. DOI:10.7554/eLife.00654. PMID 23930224. PMC: 3736543. | ||
Author | Ye F, Breslow D, Koslover E, Spakowitz A, Nelson W, Nachury M | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:50, 12 September 2013 | 8.5 s, 258 × 258 (31 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Live cell imaging of a single ciliary SSTR3 molecule. |
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Author | Ye F, Breslow D, Koslover E, Spakowitz A, Nelson W, Nachury M |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | IMCD3 cells stably expressing AP-SSTR3-GFP (pseudo-colored red) were transfected with BirA-ER to biotinylate AP-SSTR3-GFP. Biotinylated SSTR3 was detected with mSA-Alexa647 (mSA, pseudo-colored green). The tip (T) and the base (B) of the cilium are indicated. Scale bar, 2 ?m.
DOI: http://dx.doi.org/10.7554/eLife.00654.007 |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-08-06 |