File:Shiga-Toxin-Increases-Formation-of-Clathrin-Coated-Pits-through-Syk-Kinase-pone.0010944.s001.ogv
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Shiga-Toxin-Increases-Formation-of-Clathrin-Coated-Pits-through-Syk-Kinase-pone.0010944.s001.ogv (Ogg Theora video file, length 10 s, 698 × 600 pixels, 574 kbps, file size: 714 KB)
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[edit]DescriptionShiga-Toxin-Increases-Formation-of-Clathrin-Coated-Pits-through-Syk-Kinase-pone.0010944.s001.ogv |
English: Live-cell spinning-disk confocal imaging of a HeLa cell stably expressing σ2-EGFP before and after addition of StxB. The composite movie overlays two consecutive 3 min time-lapse series acquired from the same cell area before (green) and after (red) incubation for 10 min with StxB. The arrow points to a representative diffraction limited AP-2 spot appearing and disappearing within the time-lapse. Laplacian 2D and Gaussian filtering were applied to the time-lapse series to facilitate the graphic visualization [15] The last part of the movie compares the location at which new pits formed during the time series before and after addition of StxB. |
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Source | Movie S1 from Utskarpen A, Massol R, van Deurs B, Lauvrak S, Kirchhausen T, Sandvig K (2010). "Shiga Toxin Increases Formation of Clathrin-Coated Pits through Syk Kinase". PLOS ONE. DOI:10.1371/journal.pone.0010944. PMID 20668539. PMC: 2910670. | ||
Author | Utskarpen A, Massol R, van Deurs B, Lauvrak S, Kirchhausen T, Sandvig K | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:52, 17 November 2012 | 10 s, 698 × 600 (714 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Utskarpen A, Massol R, van Deurs B, Lauvrak S, Kirchhausen T, Sandvig K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live-cell spinning-disk confocal imaging of a HeLa cell stably expressing ?2-EGFP before and after addition of StxB. The composite movie overlays two consecutive 3 min time-lapse series acquired from the same cell area before (green) and after (red) incubation for 10 min with StxB. The arrow points to a representative diffraction limited AP-2 spot appearing and disappearing within the time-lapse. Laplacian 2D and Gaussian filtering were applied to the time-lapse series to facilitate the graphic visualization [15] The last part of the movie compares the location at which new pits formed during the time series before and after addition of StxB. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |