File:Septins-Regulate-Bacterial-Entry-into-Host-Cells-pone.0004196.s003.ogv
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Size of this JPG preview of this OGG file: 800 × 199 pixels. Other resolutions: 320 × 80 pixels | 640 × 159 pixels | 2,088 × 520 pixels.
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[edit]DescriptionSeptins-Regulate-Bacterial-Entry-into-Host-Cells-pone.0004196.s003.ogv |
English: Representative FRET movie of a SEPT2-depleted cell. HeLa cells treated with siRNA targeted against SEPT2 were transfected with YFP-AktPH and CFP-AktPH. Transfected siRNA-treated cells were placed on the microscope stage at 37°C and image series were collected every 15 seconds for 15 minutes. Purified InlB was added to a final concentration of 5 nM after frame 15 (i.e. time point just prior to 4 minutes) after the start of imaging. A movie for the entire timecourse of InlB stimulation for the SEPT2-depleted cell depicted in Figure 5A is here presented (i.e. frame 0 to 60), showing the collected images (phase-contrast, Akt recruitment, and FRET efficiency). Pseudocolor scale represents the range of FRET efficiency values from original signal ranging from blue (low) to red (high). |
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Source | Movie S3 from Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P (2009). "Septins Regulate Bacterial Entry into Host Cells". PLOS ONE. DOI:10.1371/journal.pone.0004196. PMID 19145258. PMC: 2626286. | ||
Author | Mostowy S, Nam Tham T, Danckaert A, Guadagnini S, Boisson-Dupuis S, Pizarro-Cerdá J, Cossart P | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:55, 17 November 2012 | 8.6 s, 2,088 × 520 (5.06 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |