File:Separase-Is-Required-for-Homolog-and-Sister-Disjunction-during-Drosophila-melanogaster-Male-Meiosis-pgen.1005996.s013.ogv

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Original file ‎(Ogg Theora video file, length 11 s, 1,648 × 880 pixels, 5.49 Mbps, file size: 7.24 MB)

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English: THR depletion by transgenic RNAi results in chromosome bridges in telophase I and II. Time-lapse analysis of progression through the first and second meiotic divisions in spermatocytes expressing EGFP-tubulin and His2Av-mRFP. Moreover, spermatocyte-specific THR depletion was induced by transgenic RNAi (thr-RNAi). Chromosome separation during anaphase I and II did not succeed in THR depleted cells resulting in abnormal telophase with unseparated chromosomes squeezed apart by the contractile furrow into two daughter cells or as a single mass into one daughter cell. As a consequence of the meiosis I segregation failure, spindles in meiosis II are often irregular due to chromatin in the division plane. Precise genotype description is given in S1 Table. Image stacks with 24 focal planes spaced by 1 μm were acquired with a time interval of 1 min. Scale bar = 10 μm.
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Source S2 Movie from Blattner A, Chaurasia S, McKee B, Lehner C (2016). "Separase Is Required for Homolog and Sister Disjunction during Drosophila melanogaster Male Meiosis, but Not for Biorientation of Sister Centromeres". PLOS Genetics. DOI:10.1371/journal.pgen.1005996. PMID 27120695. PMC: 4847790.
Author Blattner A, Chaurasia S, McKee B, Lehner C
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current01:28, 18 October 201611 s, 1,648 × 880 (7.24 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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