File:SHP-1-Phosphatase-Is-a-Critical-Regulator-in-Preventing-Natural-Killer-Cell-Self-Killing-pone.0044244.s012.ogv

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SHP-1-Phosphatase-Is-a-Critical-Regulator-in-Preventing-Natural-Killer-Cell-Self-Killing-pone.0044244.s012.ogv (Ogg Theora video file, length 59 s, 634 × 421 pixels, 940 kbps, file size: 6.57 MB)

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English: Real-time imaging of the SHP-1 knockdown NK cells recognizing each other as cellular targets. We mixed unlabelled SHP-1 shRNA transduced primary NK cells with the CMFDA-labeled normal NK-cells (green) in a coculture, and imaged cytotoxicity for up to 5 hours in the presence of 7AAD, as described previously. Unlabelled SHP-1 silenced NK cells interacted with both kinds of cells in the culture over time. However, stable conjugate formations and subsequent cytolysis were only observed when the SHP-1 silenced NK cells interacted with the SHP-1 silenced NK cells. The dying unlabelled NK cells turned red due to the uptake of 7AAD. We observed also interactions (also aggregates) of normal NK cells; however, we did not observe that the normal NK cells (green) lost their green florescence intensity and evidence of cytolysis (uptake of 7AAD) in the 5-hours live cell imaging analysis.
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Source Video S7 from Mahmood S, Kanwar N, Tran J, Zhang M, Kung S (2012). "SHP-1 Phosphatase Is a Critical Regulator in Preventing Natural Killer Cell Self-Killing". PLOS ONE. DOI:10.1371/journal.pone.0044244. PMID 22952938. PMC: 3432062.
Author Mahmood S, Kanwar N, Tran J, Zhang M, Kung S
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Date/TimeThumbnailDimensionsUserComment
current21:25, 30 October 201259 s, 634 × 421 (6.57 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 402 kbps Completed 09:47, 18 October 2018 20 s
VP9 240P 246 kbps Completed 09:47, 18 October 2018 20 s
WebM 360P 501 kbps Completed 21:31, 18 November 2012 38 s
QuickTime 144p (MJPEG) Not ready Unknown status

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