File:SHP-1-Phosphatase-Is-a-Critical-Regulator-in-Preventing-Natural-Killer-Cell-Self-Killing-pone.0044244.s011.ogv

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SHP-1-Phosphatase-Is-a-Critical-Regulator-in-Preventing-Natural-Killer-Cell-Self-Killing-pone.0044244.s011.ogv (Ogg Theora video file, length 59 s, 762 × 454 pixels, 745 kbps, file size: 5.2 MB)

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English: Real-time imaging of spontaneous killing of the SHP-1-knockdown NK cells in vitro - SHP-1 shRNA transduced NK cells. SHP-1 shRNA-transduced primary NK-cells were puromycin-selected, cultured in HBSS with 10% FCS and 50 U/ml IL-2 in the presence of 7AAD, and imaged for up to 5 hours using 10X magnification objective on a Zeiss Observer 710 station. The SHP-1 knockdown NK cells showed higher tendency for stable conjugate formation with other cells. Conjugated cells subsequently mediated self-killing and turned red due to 7AAD staining.
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Source Video S6 from Mahmood S, Kanwar N, Tran J, Zhang M, Kung S (2012). "SHP-1 Phosphatase Is a Critical Regulator in Preventing Natural Killer Cell Self-Killing". PLOS ONE. DOI:10.1371/journal.pone.0044244. PMID 22952938. PMC: 3432062.
Author Mahmood S, Kanwar N, Tran J, Zhang M, Kung S
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Date/TimeThumbnailDimensionsUserComment
current21:25, 30 October 201259 s, 762 × 454 (5.2 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 180 kbps Completed 09:47, 18 October 2018 14 s
VP9 240P 104 kbps Completed 09:47, 18 October 2018 13 s
WebM 360P 506 kbps Completed 05:08, 8 November 2012 29 s
QuickTime 144p (MJPEG) 214 kbps Completed 10:31, 14 October 2024 1.0 s

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