File:SARS-Coronavirus-Replication-Is-Supported-by-a-Reticulovesicular-Network-of-Modified-Endoplasmic-pbio.0060226.sv004.ogv
SARS-Coronavirus-Replication-Is-Supported-by-a-Reticulovesicular-Network-of-Modified-Endoplasmic-pbio.0060226.sv004.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 20 s, 320 × 240 pixels, 2.04 Mbps overall, file size: 4.93 MB)
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[edit]DescriptionSARS-Coronavirus-Replication-Is-Supported-by-a-Reticulovesicular-Network-of-Modified-Endoplasmic-pbio.0060226.sv004.ogv |
English: Animation through a z -Series of 1-nm-Thick Digital Slices (Total Depth 200 nm) of a Dual-Axis Electron Tomogram of a SARS-CoV–Infected Vero E6 Cell at 7 h p.i. The video illustrates a relatively late stage of SARS-CoV–induced membrane alterations, during which DMVs seem to merge into larger VPs (type 2). Also, various stages of virus budding can be observed at the outer membranes of these type 2 VPs. Note that clear discontinuities in the membranes of the inner vesicles can be observed, ostensibly resulting in fusion of DMV contents with each other and with the lumen of the VP. To facilitate image alignment during image reconstruction, a suspension of 10-nm gold particles was layered on top of the sections as fiducial markers. |
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Source | Video S4 from Knoops K, Kikkert M, van den Worm S, Zevenhoven-Dobbe J, van der Meer Y, Koster A, Mommaas A, Snijder E (2008). "SARS-Coronavirus Replication Is Supported by a Reticulovesicular Network of Modified Endoplasmic Reticulum". PLOS Biology. DOI:10.1371/journal.pbio.0060226. PMID 18798692. PMC: 2535663. | ||
Author | Knoops K, Kikkert M, van den Worm S, Zevenhoven-Dobbe J, van der Meer Y, Koster A, Mommaas A, Snijder E | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:30, 30 October 2012 | 20 s, 320 × 240 (4.93 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Animation through a z -Series of 1-nm-Thick Digital Slices (Total Depth 200 nm) of a Dual-Axis Electron Tomogram of a SARS-CoV?Infected Vero E6 Cell at 7 h p.i. |
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Author | Knoops K, Kikkert M, van den Worm S, Zevenhoven-Dobbe J, van der Meer Y, Koster A, Mommaas A, Snijder E |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | The video illustrates a relatively late stage of SARS-CoV?induced membrane alterations, during which DMVs seem to merge into larger VPs (type 2). Also, various stages of virus budding can be observed at the outer membranes of these type 2 VPs. Note that clear discontinuities in the membranes of the inner vesicles can be observed, ostensibly resulting in fusion of DMV contents with each other and with the lumen of the VP. To facilitate image alignment during image reconstruction, a suspension of 10-nm gold particles was layered on top of the sections as fiducial markers. |
Software used | |
Date and time of digitizing | 2008-09 |
Language | English |
- Cell lines
- Electron cryo-microscopy
- Endoplasmic reticulum
- Three-dimensional imaging videos
- Intracellular membranes
- Macromolecular substances
- Biological models
- Protein subunits
- RNA-dependent RNA-polymerase
- Viral RNA
- Videos of severe acute respiratory syndrome-related coronavirus
- Videos of viral proteins
- Viral replication