File:Role-of-Myosin-Va-in-the-Plasticity-of-the-Vertebrate-Neuromuscular-Junction-In-Vivo-pone.0003871.s009.ogv

English: Upon co-transfection of rapsyn-GFP and silMyoVa NMJs are disrupted and rapsyn-GFP widely co-localises with new AChRs in intracellular punctate structures in vivo. TA muscles of adult wildtype mice were co-transfected with rapsyn-GFP (green) and silMyoVa and simultaneously injected with BGT-AF647 (‘old receptors’, blue). Ten days later, muscles were injected with BGT-AF555 (‘new receptors’, red). Then, fluorescence signals were monitored with confocal in vivo microscopy. The movie depicts a rotating 3D projection of 53 optical slices taken at an interslice distance of 1 µm. For better visibility of the different signals, only the BGT-AF647 signal shows up initially, then the BGT-AF555 signal is shown on top after the first full turn and finally the rapsyn-GFP signal is added, as indicated in the lower left corner of the movie. The depicted frame has an actual height of 81 µm. Note, that only little ‘old receptors’ remained. These hardly overlap with ‘new receptors’ and rapsyn-GFP. Conversely, rapsyn-GFP and ‘new receptors’ extensively co-localise in numerous, intracellular punctate structures and at the rim of the putative NMJ remainder.