File:Rho-Kinase-Enhances-Contractions-of-Rat-Mesenteric-Collecting-Lymphatics-pone.0094082.s005.ogv

English: Confocal image stack of an isolated rat mesenteric collecting lymphatic labeled for ROCK2. Confocal slices were obtained in single photon mode at 2 μm intervals, which are indicated at the top left of each frame. Images of individual channels for ROCK2 (green) and nuclei, (white), and an overlay of these two channels are shown in the top row. Images of the glycocalyx (red) and smooth muscle (SM) actin (blue) channels, and an overlay of these two channels are shown in the left column. All other combinations of overlays fill out the remaining rows and columns. In the red/blue overlay, magenta pixels indicate overlap. In the green/red overlay, yellow pixels indicate overlap. In the green/blue overlay, cyan pixels indicate overlap. In the green/red/blue image, grey/white pixels indicate overlap. Overlays including nuclei are also included to help distinguish the endothelial and smooth muscle layers. Strong ROCK2 labeling is evident in the smooth muscle layer and endothelium, and is also present within the vasa vasorum on the outer surface of the lymphatic vessel. The labeling becomes weaker at the far end of the z-stack because no correction for z-distance was used in the image capturing. This vessel is representative of three separate experiments.