File:Rho-Kinase's-Role-in-Myosin-Recruitment-to-the-Equatorial-Cortex-of-Mitotic-Drosophila-S2-Cells-Is-pone.0000131.s002.ogv
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Rho-Kinase's-Role-in-Myosin-Recruitment-to-the-Equatorial-Cortex-of-Mitotic-Drosophila-S2-Cells-Is-pone.0000131.s002.ogv (Ogg Theora video file, length 4.0 s, 119 × 116 pixels, 42 kbps, file size: 21 KB)
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[edit]DescriptionRho-Kinase's-Role-in-Myosin-Recruitment-to-the-Equatorial-Cortex-of-Mitotic-Drosophila-S2-Cells-Is-pone.0000131.s002.ogv |
English: RLCT20S21-GFP is localized to the cleavage furrow. Drosophila S2 cells expressing RLCT20S21-GFP (green) and mRFP-alpha-tubulin (red) were treated with dsRNA for 5 days to deplete the endogenous RLC. Epifluorescence microscopy was used to film cells as they transitioned from metaphase into anaphase and telophase. Images were taken at 20 second intervals and movies are played at 10 frames/second. Figure 2C, row 1 contains images from this movie. |
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Source | Movie S1 from Dean S, Spudich J (2006). "Rho Kinase's Role in Myosin Recruitment to the Equatorial Cortex of Mitotic Drosophila S2 Cells Is for Myosin Regulatory Light Chain Phosphorylation". PLOS ONE. DOI:10.1371/journal.pone.0000131. PMID 17205135. PMC: 1762308. | ||
Author | Dean S, Spudich J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:29, 16 November 2012 | 4.0 s, 119 × 116 (21 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Dean S, Spudich J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | RLCT20S21-GFP is localized to the cleavage furrow. Drosophila S2 cells expressing RLCT20S21-GFP (green) and mRFP-alpha-tubulin (red) were treated with dsRNA for 5 days to deplete the endogenous RLC. Epifluorescence microscopy was used to film cells as they transitioned from metaphase into anaphase and telophase. Images were taken at 20 second intervals and movies are played at 10 frames/second. Figure 2C, row 1 contains images from this movie. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2006 |
Categories:
- Videos of cell biology
- Base sequence
- Schneider 2 cells
- Videos of Drosophila
- Drosophila proteins
- Intracellular signaling peptides and proteins
- Myosin light chains
- Myosins
- Protein-serine-threonine kinases
- RNA interference
- Recombinant fusion proteins
- Videos of Rho family of GTPases
- Rho-associated kinases
- Media from PLOS ONE