File:Ret-and-Etv4-Promote-Directed-Movements-of-Progenitor-Cells-during-Renal-Branching-Morphogenesis-pbio.1002382.s007.ogv
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[edit]DescriptionRet-and-Etv4-Promote-Directed-Movements-of-Progenitor-Cells-during-Renal-Branching-Morphogenesis-pbio.1002382.s007.ogv |
English: Lineage tracing of Ret -expressing ureteric bud tip cells in renal organ culture. A Ret-CreERT2/+; Rosa26R-tdTomato/+; Hoxb7-myrVenus/+ kidney was excised at E12.5, treated for 1 h with 1 μm 4-OH tamoxifen, washed several times with PBS and cultured in normal medium. Images were collected every 20 min. The image on the left shows the green channel (showing the entire ureteric bud epithelium) and the red channel (showing tdTomato+ cells), while the image on the right shows only the red channel. At ~10 h, red cells start to appear in the extreme tips of the ureteric bud. As the tips extend and branch, the tips remain tdTomato+, as do the newly formed trunks (indicated by arrows in last frame). The yellow numbers at bottom left show the elapsed time since the start of the movie (h:min:s on day 1 and d:h:min:s on days 2–3); there is a gap in the movie between 21h:22 min and 1d:1h:9min. |
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Source | S2 Movie from Riccio P, Cebrian C, Zong H, Hippenmeyer S, Costantini F (2016). "Ret and Etv4 Promote Directed Movements of Progenitor Cells during Renal Branching Morphogenesis". PLOS Biology. DOI:10.1371/journal.pbio.1002382. PMID 26894589. PMC: 4760680. | ||
Author | Riccio P, Cebrian C, Zong H, Hippenmeyer S, Costantini F | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 00:07, 9 March 2016 | 14 s, 1,388 × 520 (665 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Lineage tracing of Ret -expressing ureteric bud tip cells in renal organ culture. |
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Author | Riccio P, Cebrian C, Zong H, Hippenmeyer S, Costantini F |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | A Ret-CreERT2/+; Rosa26R-tdTomato/+; Hoxb7-myrVenus/+ kidney was excised at E12.5, treated for 1 h with 1 μm 4-OH tamoxifen, washed several times with PBS and cultured in normal medium. Images were collected every 20 min. The image on the left shows the green channel (showing the entire ureteric bud epithelium) and the red channel (showing tdTomato+ cells), while the image on the right shows only the red channel. At ~10 h, red cells start to appear in the extreme tips of the ureteric bud. As the tips extend and branch, the tips remain tdTomato+, as do the newly formed trunks (indicated by arrows in last frame). The yellow numbers at bottom left show the elapsed time since the start of the movie (h:min:s on day 1 and d:h:min:s on days 2–3); there is a gap in the movie between 21h:22 min and 1d:1h:9min. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-02-19 |
Categories:
- Videos of 2016
- Videos of molecular biology
- Videos of molecular biology techniques
- Videos of urinary system
- Videos of cell biology
- Cell processes
- Videos of cell physiology
- Cell movement
- Videos of developmental biology
- Branching morphogenesis
- Organism development
- Kidney development
- Genetic loci
- Animal genomics
- Mammal genetics