File:Reprogramming-of-Round-Spermatids-by-the-Germinal-Vesicle-Cytoplasm-in-Mice-pone.0078437.s001.ogv
Reprogramming-of-Round-Spermatids-by-the-Germinal-Vesicle-Cytoplasm-in-Mice-pone.0078437.s001.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 38 s, 320 × 240 pixels, 63 kbps overall, file size: 295 KB)
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[edit]DescriptionReprogramming-of-Round-Spermatids-by-the-Germinal-Vesicle-Cytoplasm-in-Mice-pone.0078437.s001.ogv |
English: Removal of GV from fully-grown oocyte. After the fully-grown GV oocytes were collected, in order to prepare cytoplasmic extracts, the entire GVs were removed using a micromanipulator in modified Hepes-CZB medium supplemented with 0.1 mg/ml dibutyryl cyclic AMP and 7.5 µg/ml cytochalasin B. The inner diameter of the pipette for nucleolus collection was approximately 5 μm. |
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Source | Movie S1 from Kong P, Zhu Y, Wang M, Li H, Chen X, Jiang M (2013). "Reprogramming of Round Spermatids by the Germinal Vesicle Cytoplasm in Mice". PLOS ONE. DOI:10.1371/journal.pone.0078437. PMID 24167624. PMC: 3805568. | ||
Author | Kong P, Zhu Y, Wang M, Li H, Chen X, Jiang M | ||
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 04:37, 15 October 2016 | 38 s, 320 × 240 (295 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kong P, Zhu Y, Wang M, Li H, Chen X, Jiang M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Removal of GV from fully-grown oocyte. After the fully-grown GV oocytes were collected, in order to prepare cytoplasmic extracts, the entire GVs were removed using a micromanipulator in modified Hepes-CZB medium supplemented with 0.1 mg/ml dibutyryl cyclic AMP and 7.5 µg/ml cytochalasin B. The inner diameter of the pipette for nucleolus collection was approximately 5 μm. |
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Language | English |