File:Real-time-tracking-of-cell-cycle-progression-during-CD8+-effector-and-memory-T-cell-differentiation-ncomms7301-s2.ogv

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Real-time-tracking-of-cell-cycle-progression-during-CD8+-effector-and-memory-T-cell-differentiation-ncomms7301-s2.ogv ‎(Ogg Theora video file, length 33 s, 485 × 491 pixels, 651 kbps, file size: 2.55 MB)

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English: Supplementary Movie 1 Time-lapse imaging of individual Fucci CD8+ T cells. Cell Trace Violet-labeled Fucci-CD8+ T cells were stimulated with plate-bound anti-CD3ε (1.0 μg/ml) and anti-CD28 mAb (0.5 μg/ml) and sorted to collect the cells in the 8th division generation. Sorted cells were seeded into microgrid arrays in the presence of rIL-2 (10 ng/ml) and subjected to time-lapse imaging every 3-4 min with 6-8 stacks at 0.8-1.2 μm (z-plane). Representative movie from 1st day of imaging showing one area from multiple positioning. Movie duration: 16 h, Objectives: 20× 0.5 NA.
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Source Video file from Kinjyo I, Qin J, Tan S, Wellard C, Mrass P, Ritchie W, Doi A, Cavanagh L, Tomura M, Sakaue-Sawano A, Kanagawa O, Miyawaki A, Hodgkin P, Weninger W (2015). "Real-time tracking of cell cycle progression during CD8+ effector and memory T-cell differentiation". Nature Communications. DOI:10.1038/ncomms7301. PMID 25709008. PMC: 4346633.
Author Kinjyo I, Qin J, Tan S, Wellard C, Mrass P, Ritchie W, Doi A, Cavanagh L, Tomura M, Sakaue-Sawano A, Kanagawa O, Miyawaki A, Hodgkin P, Weninger W
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Date/TimeThumbnailDimensionsUserComment
current20:05, 1 November 201633 s, 485 × 491 (2.55 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 253 kbps Completed 03:48, 17 October 2018 11 s
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VP9 240P 52 kbps Completed 03:48, 17 October 2018 6.0 s
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Streaming 144p (MJPEG) 332 kbps Completed 15:40, 18 November 2023 1.0 s

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