File:Rapid-and-Direct-Transport-of-Cell-Surface-APP-to-the-Lysosome-defines-a-novel-selective-pathway-1756-6606-3-11-S2.ogv
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Rapid-and-Direct-Transport-of-Cell-Surface-APP-to-the-Lysosome-defines-a-novel-selective-pathway-1756-6606-3-11-S2.ogv (Ogg Theora video file, length 25 s, 320 × 240 pixels, 179 kbps, file size: 545 KB)
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[edit]DescriptionRapid-and-Direct-Transport-of-Cell-Surface-APP-to-the-Lysosome-defines-a-novel-selective-pathway-1756-6606-3-11-S2.ogv |
English: Image stack of an immunostained mouse neuron demonstrating APP in the lysosome. Cultured mouse neurons were fixed and immunolabeled with anti-APP (green) and anti-LAMP1 antibodies (red) and Hoechst nuclear stain (blue). Image stack was acquired on a Zeiss LSM510 confocal microscope. The first segment shows βAPP-CFP in green. The second segment of the video shows LAMP1-mRFP overlaid in red. In the third segment, a channel showing the colocalization of the brightest 2% of red and green pixels is overlaid in white. QuickTime video was generated using Imaris 6.1.5 software to perform volume rendering and animation. |
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Source | Lorenzen A, Samosh J, Vandewark K, Anborgh P, Seah C, Magalhaes A, Cregan S, Ferguson S, Pasternak S (2010). "Rapid and Direct Transport of Cell Surface APP to the Lysosome defines a novel selective pathway". Molecular Brain. DOI:10.1186/1756-6606-3-11. PMID 20409323. PMC: 2868040. | ||
Author | Lorenzen A, Samosh J, Vandewark K, Anborgh P, Seah C, Magalhaes A, Cregan S, Ferguson S, Pasternak S | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 00:08, 20 November 2012 | 25 s, 320 × 240 (545 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Lorenzen A, Samosh J, Vandewark K, Anborgh P, Seah C, Magalhaes A, Cregan S, Ferguson S, Pasternak S |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Image stack of an immunostained mouse neuron demonstrating APP in the lysosome. Cultured mouse neurons were fixed and immunolabeled with anti-APP (green) and anti-LAMP1 antibodies (red) and Hoechst nuclear stain (blue). Image stack was acquired on a Zeiss LSM510 confocal microscope. The first segment shows ?APP-CFP in green. The second segment of the video shows LAMP1-mRFP overlaid in red. In the third segment, a channel showing the colocalization of the brightest 2% of red and green pixels is overlaid in white. QuickTime video was generated using Imaris 6.1.5 software to perform volume rendering and animation. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2010 |