File:Rapid-Screening-of-Gene-Function-by-Systemic-Delivery-of-Morpholino-Oligonucleotides-to-Live-Mouse-pone.0114932.s001.ogv
Rapid-Screening-of-Gene-Function-by-Systemic-Delivery-of-Morpholino-Oligonucleotides-to-Live-Mouse-pone.0114932.s001.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 28 s, 630 × 540 pixels, 350 kbps overall, file size: 1.15 MB)
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[edit]DescriptionRapid-Screening-of-Gene-Function-by-Systemic-Delivery-of-Morpholino-Oligonucleotides-to-Live-Mouse-pone.0114932.s001.ogv |
English: Demonstration of heart injection of constructs in 11.5 dpc embryo. This video demonstrates the injection of a construct (marked by blue dye) into the left ventricle of the beating embryo heart at 11.5 dpc. After several heartbeats the dye can be seen in more distal parts of the embryo and finally in the head vein indicating successful injection. After injection the embryo is incubated with the heart still beating for 30 min before dissection for organ culture. For more detailed information see Fig. 1 and Materials and Methods. |
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Source | S1 Video from McClelland K, Wainwright E, Bowles J, Koopman P (2015). "Rapid Screening of Gene Function by Systemic Delivery of Morpholino Oligonucleotides to Live Mouse Embryos". PLOS ONE. DOI:10.1371/journal.pone.0114932. PMID 25629157. PMC: 4309589. | ||
Author | McClelland K, Wainwright E, Bowles J, Koopman P | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 21:27, 7 February 2015 | 28 s, 630 × 540 (1.15 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Demonstration of heart injection of constructs in 11.5 dpc embryo. |
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Author | McClelland K, Wainwright E, Bowles J, Koopman P |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | This video demonstrates the injection of a construct (marked by blue dye) into the left ventricle of the beating embryo heart at 11.5 dpc. After several heartbeats the dye can be seen in more distal parts of the embryo and finally in the head vein indicating successful injection. After injection the embryo is incubated with the heart still beating for 30 min before dissection for organ culture. For more detailed information see Fig. 1 and Materials and Methods. |
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Language | English |