File:Rac1-Dependent-Collective-Cell-Migration-Is-Required-for-Specification-of-the-Anterior-Posterior-pbio.1000442.s013.ogv

English: AVE cells send long lamellipodia that are spatially and temporally coordinated. A wild-type embryo was dissected on e5.6 at mid-afternoon, and imaged for 6 h. Images were taken every 10 min. At each time point, a stack of 39 slices was taken, at 2 µm per slice. Extended focus images have been aligned to correct for the shift of the embryo, using the distal border as a reference point. Individual cells were pseudocolored to visualize their trajectories. Cells were tracked using individual confocal images of each stack to increase the accuracy of the cell contours.