File:Quantifying-Exocytosis-by-Combination-of-Membrane-Capacitance-Measurements-and-Total-Internal-pone.0000505.s001.ogv
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Quantifying-Exocytosis-by-Combination-of-Membrane-Capacitance-Measurements-and-Total-Internal-pone.0000505.s001.ogv (Ogg Theora video file, length 14 s, 330 × 378 pixels, 126 kbps, file size: 215 KB)
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[edit]DescriptionQuantifying-Exocytosis-by-Combination-of-Membrane-Capacitance-Measurements-and-Total-Internal-pone.0000505.s001.ogv |
English: TIRF-Microscopy (top) and membrane capacitance (bottom) recording of the cell shown in Figure 1a. This cell was stimulated for 2 s by depolarizing pulses and is shown 4 s prior to and 8.5 s after stimulation. Secretion of vesicles seen as a sharp decrease of fluorescence of individual spots (highlighted by white arrows) occurs simultaneously to the increase in membrane capacitance and only during the stimulation period (marked in red). Before and after stimulation vesicles may enter or leave the evanescent field as can be seen by a slow increase or decrease of individual fluorescent spots. Acquisition and display rate are 5 Hz. |
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Source | Movie S1 from Becherer U, Pasche M, Nofal S, Hof D, Matti U, Rettig J (2007). "Quantifying Exocytosis by Combination of Membrane Capacitance Measurements and Total Internal Reflection Fluorescence Microscopy in Chromaffin Cells". PLOS ONE. DOI:10.1371/journal.pone.0000505. PMID 17551585. PMC: 1876815. | ||
Author | Becherer U, Pasche M, Nofal S, Hof D, Matti U, Rettig J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 15:33, 30 October 2012 | 14 s, 330 × 378 (215 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Becherer U, Pasche M, Nofal S, Hof D, Matti U, Rettig J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | TIRF-Microscopy (top) and membrane capacitance (bottom) recording of the cell shown in Figure 1a. This cell was stimulated for 2 s by depolarizing pulses and is shown 4 s prior to and 8.5 s after stimulation. Secretion of vesicles seen as a sharp decrease of fluorescence of individual spots (highlighted by white arrows) occurs simultaneously to the increase in membrane capacitance and only during the stimulation period (marked in red). Before and after stimulation vesicles may enter or leave the evanescent field as can be seen by a slow increase or decrease of individual fluorescent spots. Acquisition and display rate are 5 Hz. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2007 |