File:Quaking-promotes-monocyte-differentiation-into-pro-atherogenic-macrophages-by-controlling-pre-mRNA-ncomms10846-s3.ogv
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[edit]DescriptionQuaking-promotes-monocyte-differentiation-into-pro-atherogenic-macrophages-by-controlling-pre-mRNA-ncomms10846-s3.ogv |
English: Supplementary Movie 2 Initial adhesion of sh-QKI THP-1 ‘monocytes' in an in vitro cell perfusion system. Glass coverslips were coated with type I collagen, after which the system was perfused with platelet-rich plasma for 10 minutes, leading to the deposition of effector molecules to which the monocyte can adhere. This movie displays the perfusion of sh-QKI THP-1 ‘monocytes' over this bio-active substrate, leading to a reduction in their attachment to the surface as compared to that seen for sh-Cont THP-1 ‘monocytes' in Supplementary Movie 1. Total cellular perfusion time was 5 minutes with a flow rate of 1 dyne/cm2. The movie is representative of at least three perfusions. |
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Source | Video file from de Bruin R, Shiue L, Prins J, de Boer H, Singh A, Fagg W, van Gils J, Duijs J, Katzman S, Kraaijeveld A, Böhringer S, Leung W, Kielbasa S, Donahue J, van der Zande P, Sijbom R, van Alem C, Bot I, van Kooten C, Jukema J, Van Esch H, Rabelink T, Kazan H, Biessen E, Ares Jr. M, van Zonneveld A, van der Veer E (2016). "Quaking promotes monocyte differentiation into pro-atherogenic macrophages by controlling pre-mRNA splicing and gene expression". Nature Communications. DOI:10.1038/ncomms10846. PMID 27029405. PMC: 4821877. | ||
Author | de Bruin R, Shiue L, Prins J, de Boer H, Singh A, Fagg W, van Gils J, Duijs J, Katzman S, Kraaijeveld A, Böhringer S, Leung W, Kielbasa S, Donahue J, van der Zande P, Sijbom R, van Alem C, Bot I, van Kooten C, Jukema J, Van Esch H, Rabelink T, Kazan H, Biessen E, Ares Jr. M, van Zonneveld A, van der Veer E | ||
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current | 12:17, 28 October 2016 | 15 s, 964 × 720 (433 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 2 |
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Author | de Bruin R, Shiue L, Prins J, de Boer H, Singh A, Fagg W, van Gils J, Duijs J, Katzman S, Kraaijeveld A, Böhringer S, Leung W, Kielbasa S, Donahue J, van der Zande P, Sijbom R, van Alem C, Bot I, van Kooten C, Jukema J, Van Esch H, Rabelink T, Kazan H, Biessen E, Ares Jr. M, van Zonneveld A, van der Veer E |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Initial adhesion of sh-QKI THP-1 ‘monocytes' in an in vitro cell perfusion system. Glass coverslips were coated with type I collagen, after which the system was perfused with platelet-rich plasma for 10 minutes, leading to the deposition of effector molecules to which the monocyte can adhere. This movie displays the perfusion of sh-QKI THP-1 ‘monocytes' over this bio-active substrate, leading to a reduction in their attachment to the surface as compared to that seen for sh-Cont THP-1 ‘monocytes' in Supplementary Movie 1. Total cellular perfusion time was 5 minutes with a flow rate of 1 dyne/cm2. The movie is representative of at least three perfusions. |
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Date and time of digitizing | 2016-03-31 |