File:Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s016.ogv
Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s016.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 5.4 s, 410 × 152 pixels, 249 kbps overall, file size: 164 KB)
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[edit]DescriptionPseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s016.ogv |
English: Transient transfection with pCrkI/R38K is sufficient to cause cytotoxicity in Crk -/- cells. Crk-/- cells were transfected with either pIRES2-GFP empty vector, pCrkI-GFP, pCrkI/R38K-GFP, or pCrkI/R38K,W170K-GFP. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide, an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that only pCrkI/R38K transfected cells pick up PI. |
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Source | S7 Movie from Wood S, Goldufsky J, Shafikhani S (2015). "Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin". PLOS Pathogens. DOI:10.1371/journal.ppat.1004934. PMID 26020630. PMC: 4447348. | ||
Author | Wood S, Goldufsky J, Shafikhani S | ||
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![]() ![]() This file is licensed under the Creative Commons Attribution 4.0 International license.
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 20:27, 10 June 2015 | 5.4 s, 410 × 152 (164 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Transient transfection with pCrkI/R38K is sufficient to cause cytotoxicity in Crk -/- cells. |
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Author | Wood S, Goldufsky J, Shafikhani S |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Crk-/- cells were transfected with either pIRES2-GFP empty vector, pCrkI-GFP, pCrkI/R38K-GFP, or pCrkI/R38K,W170K-GFP. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide, an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that only pCrkI/R38K transfected cells pick up PI. |
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Language | English |