File:Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s015.ogv

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Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s015.ogv (Ogg Theora video file, length 4.1 s, 320 × 152 pixels, 290 kbps, file size: 144 KB)

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English: Crk -/- cells, complemented with CrkI, are susceptible to ExoT-induced cytotoxicity. Crk-/- cells were transfected with the pCrkI-GFP vector on consecutive days to increase transfection efficiency. 24 hr after final transfection, transfected cells were infected with PA103ΔexoU (ΔU), PA103∆exoU/exoT(R149K) (∆U/T(G-A+)), or the T3SS mutant PA103 pscJ::Tn5 (pscJ) at MOI ~10. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide (PI), an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that transfected cells (green) do not pick up PI.
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Source S6 Movie from Wood S, Goldufsky J, Shafikhani S (2015). "Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin". PLOS Pathogens. DOI:10.1371/journal.ppat.1004934. PMID 26020630. PMC: 4447348.
Author Wood S, Goldufsky J, Shafikhani S
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Date/TimeThumbnailDimensionsUserComment
current20:26, 10 June 20154.1 s, 320 × 152 (144 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 122 kbps Completed 19:16, 15 July 2024 2.0 s
WebM 360P 166 kbps Completed 06:54, 2 December 2023 1.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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