File:Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s014.ogv

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Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s014.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 4.1 s, 320 × 162 pixels, 302 kbps overall, file size: 150 KB)

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English: Crk -/- cells, complemented with GFP are resistant to ExoT-induced cytotoxicity. Crk-/- cells were transfected with the pIRES2-GFP empty vector on consecutive days to increase transfection efficiency. 24 hr after final transfection, transfected cells were infected with PA103ΔexoU (ΔU), PA103∆exoU/exoT(R149K) (∆U/T(G-A+)), or the T3SS mutant PA103 pscJ::Tn5 (pscJ) at MOI ~10. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide (PI), an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that transfected cells (green) do not pick up PI.
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Source S5 Movie from Wood S, Goldufsky J, Shafikhani S (2015). "Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin". PLOS Pathogens. DOI:10.1371/journal.ppat.1004934. PMID 26020630. PMC: 4447348.
Author Wood S, Goldufsky J, Shafikhani S
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Date/TimeThumbnailDimensionsUserComment
current20:26, 10 June 20154.1 s, 320 × 162 (150 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 132 kbps Completed 19:16, 15 July 2024 3.0 s
WebM 360P 572 kbps Completed 06:54, 2 December 2023 1.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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