File:Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s012.ogv

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Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s012.ogv (Ogg Theora video file, length 5.4 s, 714 × 172 pixels, 2.65 Mbps, file size: 1.71 MB)

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English: HeLa S3 cells are resistant to ExoT and ADPRT mediated cytotoxicity. HeLa S3 cells were infected with either PA103ΔexoU (ΔU), PA103∆exoU/exoT(R149K) (∆U/T(G-A+)), or the T3SS mutant PA103 pscJ::Tn5 (pscJ) at MOI ~10 or given PBS (Mock). Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide (PI), an impermeant nuclear dye, identifies dying cells (red). Note the lack of cytotoxicity caused by ΔU and ∆U/T(G-A+).
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Source S3 Movie from Wood S, Goldufsky J, Shafikhani S (2015). "Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin". PLOS Pathogens. DOI:10.1371/journal.ppat.1004934. PMID 26020630. PMC: 4447348.
Author Wood S, Goldufsky J, Shafikhani S
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Date/TimeThumbnailDimensionsUserComment
current20:26, 10 June 20155.4 s, 714 × 172 (1.71 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 360P 610 kbps Completed 09:26, 16 October 2018 7.0 s
VP9 240P 279 kbps Completed 09:26, 16 October 2018 4.0 s
WebM 360P 514 kbps Completed 08:25, 2 December 2016 7.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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