File:Protein-Complexes-and-Proteolytic-Activation-of-the-Cell-Wall-Hydrolase-RipA-Regulate-Septal-ppat.1003197.s011.ogv

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Original file ‎(Ogg Theora video file, length 25 s, 1,024 × 1,024 pixels, 387 kbps, file size: 1.14 MB)

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English: RipASm C408A cells predominantly chain but also occasionally lyse. M. smegmatis were grown on an agar pad, containing the inducer aTc in order to overexpress the catalytic mutant of RipASm (C408A). A cytosolic GFP reporter was used to identify cells inducing RipASm C408A expression and visualize chains of cells. Images were captured every 10 minutes, and a time-lapse movie was generated. Lysis of specific cells within the chain can be observed by localized loss of GFP signal between frames.
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Source Video S2 from Chao M, Kieser K, Minami S, Mavrici D, Aldridge B, Fortune S, Alber T, Rubin E (2013). "Protein Complexes and Proteolytic Activation of the Cell Wall Hydrolase RipA Regulate Septal Resolution in Mycobacteria". PLOS Pathogens. DOI:10.1371/journal.ppat.1003197. PMID 23468634. PMC: 3585148.
Author Chao M, Kieser K, Minami S, Mavrici D, Aldridge B, Fortune S, Alber T, Rubin E
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current19:42, 8 March 201325 s, 1,024 × 1,024 (1.14 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 84 kbps Completed 07:17, 16 October 2018 8.0 s
Streaming 720p (VP9) Not ready Unknown status
VP9 480P 37 kbps Completed 07:17, 16 October 2018 4.0 s
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VP9 360P 21 kbps Completed 07:17, 16 October 2018 5.0 s
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VP9 240P 14 kbps Completed 07:17, 16 October 2018 2.0 s
Streaming 240p (VP9) 14 kbps Completed 23:53, 15 December 2023 1.0 s
WebM 360P 115 kbps Completed 19:44, 8 March 2013 6.0 s
Streaming 144p (MJPEG) 64 kbps Completed 16:12, 20 November 2023 1.0 s

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