File:Problems associated with the need for standardization in clinical spectrophotometric and fluorometric measurements (IA jresv76An5p491).pdf

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Problems associated with the need for standardization in clinical spectrophotometric and fluorometric measurements ( )
Author	Penton, James R. Widdowson, Graham M. Williams, George Z.
Title	Problems associated with the need for standardization in clinical spectrophotometric and fluorometric measurements
Volume	76A
Publisher	National Bureau of Standards
Description	Journal of Research of the National Bureau of Standards Subjects: Clinical standards; standard reference materials; standardization, spectrofluorometric; standardization, spectrophotometric
Language	English
Publication date	1972 publication_date QS:P577,+1972-00-00T00:00:00Z/9
Current location	IA Collections: NISTJournalofResearch; NISTresearchlibrary; fedlink
Accession number	jresv76An5p491
Source	Internet Archive identifier: jresv76An5p491 https://archive.org/download/jresv76An5p491/jresv76An5p491.pdf
Permission (Reusing this file)	The Journal of Research of the National Institute of Standards and Technology is a publication of the U.S. Government. The papers are in the public domain and are not subject to copyright in the United States. However, please pay special attention to the

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Short title	Problems associated with the need for standardization in clinical spectrophotometric and fluorometric measurements
Image title	There is a growing demand in clinical chemistry for analyses to be performed in a manner allowing comparisons of results among laboratories and, from time to time, in the same laboratory. Reliable comparability requires adequate procedures of standardization for spectrophotometric and fluorometric instruments and methods. Problems with chemical and instrumental standardization are discussed. For assays where the substance to be measured is available in suitable form, primary chemical standardization is justifiably popular. Relatively unsophisticated instrumentation can be used to compare measurements of unknown samples with such standards. Because primary standards meeting all necessary criteria are not available for many assays of clinical significance, standardization must depend on precision and accuracy of the instrumentation used, and on accurately compiled values of chemical-optical properties for the materials of interest. The task of compilation is outside the capability of the routine laboratory and should be provided by a reliable central agency. If an individual laboratory is to use the agency's compiled values, that laboratory must have available precise, accurate and reasonably inexpensive instrumentation along with reliable absorbance, fluorescence, and wavelength calibration standards.
Author	Penton
Keywords	Clinical standards; standard reference materials; standardization; spectrofluorometric; standardization; spectrophotometric
Software used	Adobe Acrobat 9.0
Conversion program	Adobe Acrobat 9.13 Paper Capture Plug-in
Encrypted	no
Page size	531.24 x 726.24 pts 530.28 x 726.24 pts 530.76 x 725.76 pts 531.24 x 725.76 pts
Version of PDF format	1.4

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