File:Prevention-of-LPS-Induced-Microglia-Activation-Cytokine-Production-and-Sickness-Behavior-with-TLR4-pone.0060388.s002.ogv
Prevention-of-LPS-Induced-Microglia-Activation-Cytokine-Production-and-Sickness-Behavior-with-TLR4-pone.0060388.s002.ogv (Ogg Theora video file, length 22 s, 654 × 480 pixels, 418 kbps, file size: 1.09 MB)
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[edit]DescriptionPrevention-of-LPS-Induced-Microglia-Activation-Cytokine-Production-and-Sickness-Behavior-with-TLR4-pone.0060388.s002.ogv |
English: Live 2-photon imaging of the effects of LPS on microglia morphology, and blockade of LPS-induced microglia morphology changes by treatment with Tat-MyD88. Under control conditions, microglia in acutely prepared brain slices exhibit the typical ramified morphology characterized by numerous long branches, and multiple filopodia. Within 10 min following LPS treatment, we observed the first indications of morphology change, and by 40 minutes, the majority of branches were lost and the cells were amoeboid. The amoeboid morphology of microglia persisted throughout the remainder of imaging (80 min). In comparison, when slices were preincubated with Tat-MyD88, the transition from ramified to amoeboid characterized by branch loss was not observed at either 40 or 80 minutes following LPS treatment. |
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Source | Movie S1 from Hines D, Choi H, Hines R, Phillips A, MacVicar B (2013). "Prevention of LPS-Induced Microglia Activation, Cytokine Production and Sickness Behavior with TLR4 Receptor Interfering Peptides". PLOS ONE. DOI:10.1371/journal.pone.0060388. PMID 23555964. PMC: 3610686. | ||
Author | Hines D, Choi H, Hines R, Phillips A, MacVicar B | ||
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current | 03:37, 7 April 2013 | 22 s, 654 × 480 (1.09 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Hines D, Choi H, Hines R, Phillips A, MacVicar B |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live 2-photon imaging of the effects of LPS on microglia morphology, and blockade of LPS-induced microglia morphology changes by treatment with Tat-MyD88. Under control conditions, microglia in acutely prepared brain slices exhibit the typical ramified morphology characterized by numerous long branches, and multiple filopodia. Within 10 min following LPS treatment, we observed the first indications of morphology change, and by 40 minutes, the majority of branches were lost and the cells were amoeboid. The amoeboid morphology of microglia persisted throughout the remainder of imaging (80 min). In comparison, when slices were preincubated with Tat-MyD88, the transition from ramified to amoeboid characterized by branch loss was not observed at either 40 or 80 minutes following LPS treatment. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |