File:Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s008.ogv
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Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s008.ogv (Ogg Theora video file, length 33 s, 575 × 574 pixels, 744 kbps, file size: 2.89 MB)
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[edit]DescriptionPremitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s008.ogv |
English: 4-Dimensional imaging of rolling spindles: projection. HeLa-H2B-GFP cells transfected with pooled siRNAs against CENP-W were imaged by 3-dimensional time-lapse microscopy beginning 48 h after transfection, with frames collected every 5 min. This sequence shows a projected image superimposed on a transmitted light image of cells. Two rolling cells are detailed. In one (first), the metaphase plate rotates in a continuous motion through almost 180°. In another, the metaphase plate rotates 90° with respect to the optical axis. |
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Source | Video S2 from Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K (2011). "Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State". PLOS Biology. DOI:10.1371/journal.pbio.1001082. PMID 21695110. PMC: 3114758. | ||
Author | Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:25, 14 November 2012 | 33 s, 575 × 574 (2.89 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-06 |