File:Premitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s007.ogv
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[edit]DescriptionPremitotic-Assembly-of-Human-CENPs--T-and--W-Switches-Centromeric-Chromatin-to-a-Mitotic-State-pbio.1001082.s007.ogv |
English: CENP-W knockdown induces profound mitotic delays and induces chromosome/spindle rolling in cells. HeLa-H2B-GFP cells were transfected with pooled siRNAs against CENP-W and imaged for 12 h beginning 48-h transfection at a rate of 20 frames/hour. Numerous cells are observed entering mitosis and spending extended time in a prometaphase-like state with uncongressed chromosomes. Many cells exhibit a pronounced “rolling” phenotype (see upper right quadrant) that can initiate very soon after the onset of mitosis. See Figure 2B for a quantitative interpretation of these data. |
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Source | Video S1 from Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K (2011). "Premitotic Assembly of Human CENPs -T and -W Switches Centromeric Chromatin to a Mitotic State". PLOS Biology. DOI:10.1371/journal.pbio.1001082. PMID 21695110. PMC: 3114758. | ||
Author | Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 22:25, 14 November 2012 | 22 s, 767 × 762 (1.99 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Prendergast L, van Vuuren C, Kaczmarczyk A, Doering V, Hellwig D, Quinn N, Hoischen C, Diekmann S, Sullivan K |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | CENP-W knockdown induces profound mitotic delays and induces chromosome/spindle rolling in cells. HeLa-H2B-GFP cells were transfected with pooled siRNAs against CENP-W and imaged for 12 h beginning 48-h transfection at a rate of 20 frames/hour. Numerous cells are observed entering mitosis and spending extended time in a prometaphase-like state with uncongressed chromosomes. Many cells exhibit a pronounced ?rolling? phenotype (see upper right quadrant) that can initiate very soon after the onset of mitosis. See Figure 2B for a quantitative interpretation of these data. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-06 |