File:Polarized-Exocytosis-Induces-Compensatory-Endocytosis-by-Sec4p-Regulated-Cortical-Actin-pbio.1002534.s004.ogv

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Polarized-Exocytosis-Induces-Compensatory-Endocytosis-by-Sec4p-Regulated-Cortical-Actin-pbio.1002534.s004.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 16 s, 640 × 480 pixels, 188 kbps overall, file size: 374 KB)

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English: Photobleaching enables single particle tracking of GFP-Sec4p at the cell cortex. Time-lapse videos of two representative wild-type cells (BY4741) expressing both GFP-Sec4p and Abp1p-RFP. Large white circles indicate the photobleached region (bud membrane), and asterisks indicate the duration of the laser burst. Green arrowheads indicate cortical GFP-Sec4p particles, RFP-Abp1p is indicated by red arrowheads, and yellow arrowheads indicate the transitional overlap of GFP-Sec4p and RFP-Abp1p fluorescence. Total acquisition time is 90 s edited and compressed to play at 5 frames/s (for technical reasons, 3 frames during laser photobleaching were excluded).
Date
Source S1 Movie from Johansen J, Alfaro G, Beh C (2016). "Polarized Exocytosis Induces Compensatory Endocytosis by Sec4p-Regulated Cortical Actin Polymerization". PLOS Biology. DOI:10.1371/journal.pbio.1002534. PMID 27526190. PMC: 4985162.
Author Johansen J, Alfaro G, Beh C
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current03:46, 25 September 201616 s, 640 × 480 (374 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 480P 105 kbps Completed 15:14, 17 February 2023 6.0 s
VP9 360P 62 kbps Completed 15:14, 17 February 2023 8.0 s
VP9 240P 40 kbps Completed 15:14, 17 February 2023 5.0 s
WebM 360P 250 kbps Completed 15:14, 17 February 2023 4.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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