File:Polarized-Cell-Division-of-Chlamydia-trachomatis-ppat.1005822.s005.ogv

English: 3-D animation illustrating the spatial organization of MOMP, Hsp60, and DNA in Chlamydia at an intermediate stage of the polarized cell division process. HeLa cells infected with C. trachomatis were fixed at 11 hours post-infection. Following permeabilization, the cells were incubated with rabbit polyclonal antibodies against Hsp60 (blue) and goat polyclonal antibodies against MOMP (green) followed by donkey anti-rabbit IgG conjugated to Alexa Fluor 568 and donkey anti-goat IgG conjugated to Alexa Fluor 488. The cells were stained Hoechst 33342 (red) prior to analysis by epifluorescent microscopy. The movie is an animation of a 3 dimensional projection of the z-slices that extended above and below the cell. The stack size was 3.5μ in total. UniProtKB gene accession numbers–C. trachomatis Hsp60, 60 kDa chaperonin GroEL—A0A0H3MAQ6; C. trachomatis EF-Tu, elongation factor Tu—B0B7N8; C. trachomatis MOMP, major outer membrane porin—P06597. Accession numbers are for C. trachomatis serovar L2 (strain 434/Bu).