File:Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s003.ogv
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Plexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s003.ogv (Ogg Theora video file, length 6.2 s, 800 × 600 pixels, 835 kbps, file size: 632 KB)
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[edit]DescriptionPlexin-B2-Negatively-Regulates-Macrophage-Motility-Rac-and-Cdc42-Activation-pone.0024795.s003.ogv |
English: Live cell video of cell motility of wild type macrophages. Representative cell movement videos over a 2.5 hour time period. 1×104 cells were plated on glass bottom dishes (MatTek Ashland, MA P35G-1.5-10-C) in complete DMEM, rested overnight, and treated with sham (warmed DMEM). Cells were imaged on a Nikon Biostation (Belmont, CA) with a 20× objective at ten different points. Images were collected every 5 minutes for a total time of 2.5 hours following sham treatment. Three separate movies were filmed and 45 cells scored for velocity. Cell velocity was analyzed using manual tracking in ImageJ [76]. The experiment was repeated three separate times using cells from different mice in each experiment. |
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Source | Video S1 from Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J (2011). "Plexin-B2 Negatively Regulates Macrophage Motility, Rac, and Cdc42 Activation". PLOS ONE. DOI:10.1371/journal.pone.0024795. PMID 21966369. PMC: 3179467. | ||
Author | Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 03:29, 14 November 2012 | 6.2 s, 800 × 600 (632 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Roney K, O'Connor B, Wen H, Holl E, Guthrie E, Davis B, Jones S, Jha S, Sharek L, Garcia-Mata R, Bear J, Ting J |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |
Categories:
- Videos of leukocytes
- Innate immunity
- CD45 antigens
- Western blot
- Bone marrow cells
- Cell movement
- Cell transplantation
- Videos of cultured cells
- Videos of cytokines
- Flow cytometry
- Videos of livers
- Videos of macrophages
- Congenic mice
- Inbred C57BL mice
- Knockout mice
- Nerve tissue proteins
- Protein binding
- Videos of spleen
- Videos of CDC42
- Rac1 GTP-binding protein
- Media from PLOS ONE