File:Photoswitching-Free-FRAP-Analysis-with-a-Genetically-Encoded-Fluorescent-Tag-pone.0107730.s001.ogv

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Photoswitching-Free-FRAP-Analysis-with-a-Genetically-Encoded-Fluorescent-Tag-pone.0107730.s001.ogv (Ogg Theora video file, length 24 s, 300 × 300 pixels, 1.42 Mbps, file size: 4.05 MB)

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English: Representative movie of a whole nuclear photobleach of histone H2B. The entire nucleus of HeLa cells expressing H2B-Halo-TMR (surrounded by a yellow line) was photobleached 4 sec after the start of image acquisition. The 4 sec of pre-bleach and the 20 sec of post-bleach images are shown at the same frame rate (30 Hz). There is virtually no recovery in fluorescence intensity inside the nucleus after the photobleaching. The corresponding fluorescence recovery curve is shown in Fig. 2B as a green curve.
Date
Source Movie S1 from Morisaki T, McNally J (2014). "Photoswitching-Free FRAP Analysis with a Genetically Encoded Fluorescent Tag". PLOS ONE. DOI:10.1371/journal.pone.0107730. PMID 25233348. PMC: 4169462.
Author Morisaki T, McNally J
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Creative Commons CC-Zero This file is made available under the Creative Commons CC0 1.0 Universal Public Domain Dedication.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current22:50, 10 December 201424 s, 300 × 300 (4.05 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 139 kbps Completed 17:25, 13 October 2018 18 s
WebM 360P 370 kbps Completed 05:26, 2 December 2023 3.0 s
QuickTime 144p (MJPEG) Not ready Unknown status

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